The present studies were carried out in order to develop techniques for clonal propagation of tulip bulb by means of in vitro culture. Explants of scales, meristems and ovaries were taken from three commercial varieties; i.e., ´Golden Apeldoorn´ ´Merry Widow´ and ´Red Matador´ which were cultured on Murashige-Skoog´s media added auxin and kinetin to determine the most favorable concentrations for callus and/or organ formation.
Among experimental varieties, N and K content in the excised bulb scale explants was the lowest in ´Merry Widow´, whereas the Ca content was the highest in ´Red Matador´.
In ovary culture, the growth of ovule was the most effective on the medium supplemented with 0.01∼2.0㎎/ℓ kinetin except ´Merry Widow´.
Callus formation of cultured explants taken from ´Golden Apeldoorn´ and ´Red Matador´ was induced on the medium containing 10㎎/ℓ 2,4-D or kinetin and IAA at a appropriate concentration. But the callus proliferation on the medium added kinetin and IAA was less effective in any case.
The scale tissue of ´Red Matador´ and ´Golden Apledoorn´ formed 50% and 60% adventitious roots respectively, when they were cultured on the medium supplemented with 2.0㎎/ℓ 2,4-D.
The scale tissue of ´Golden Apeldoorn´ cultured on the medium containing 10㎎/ℓ kinetin was capable of producing the bulb-like protuberances. In the case of ´Red Matador´, the protuberances were formed on the medium with 1.0㎎/ℓ 2,4-D.
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